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Figure 1 | Cell Communication and Signaling

Figure 1

From: Nuclear trafficking of secreted factors and cell-surface receptors: new pathways to regulate cell proliferation and differentiation, and involvement in cancers

Figure 1

A NLS is located in the CT domain of the CCN3 protein. A) Conservation of the primary structure of this Arg/Lys-rich NLS in the human CCN proteins at the beginning of CT module. Basic amino acids are colored in red and marked with an asterisk in the human CCN3 sequence. These Arginine and Lysine residues are colored in blue when they are not conserved in the other CCN proteins. Replacement of a basic residue by another is represented in orange. Other non-conserved amino acids are colored in grey. B) Human G59 glioblastoma cells transiently transfected with GFP-NLS constructs. C) Baby Hamster Kidney 21 (BHK21) cells transiently transfected with CCN3 constructs (similar strategy of construction as the one described in [5]. GFP and DAPI autofluorescence were detected with epifluorescence under appropriate filters. CCN3 was detected by immunostaining with K19M antibodies [122].

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Cell Communication and Signaling

ISSN: 1478-811X