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Figure 3 | Cell Communication and Signaling

Figure 3

From: Anti-lipid phosphate phosphohydrolase-3 (LPP3) antibody inhibits bFGF- and VEGF-induced capillary morphogenesis of endothelial cells

Figure 3

Tumor endothelium express LPP3 protein alongside VEGF. Paraffin-embedded angioma (A-C) and hemangioma (D-I) tumor tissue sections (4 μm) were subjected to antigen retrieval, and sequentially incubated with the indicated antibodies. After washing with PBS, sections were incubated with donkey anti-goat/rabbit IgG conjugated to Texas-red (red) and goat anti-mouse IgG conjugated to FITC (green). C, F, and I images represent overlays of A, B; D, E; and G, H respectively. Images were captured below saturation level. Merged yellow represents co-expression. Data shown are representative of those obtained in at least three separate experiments, with similar results. (L, lumen; Magnification, 100×; Bars, 50 μM).

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