Figure 2

LPP3 is a cell surface antigen. A) HUVECs (passage 4) were serum-starved for 6 h, thereafter, left untreated (-) or treated with VEGF¹⁶⁵ (100 ng/ml) for 6 h and subjected to fluorescent activated cell sorting (FACS) using indicated antibodies. B) Schematic representation of human LPP3 protein showing 6-transmembrane organization. Proposed 3 extracellular loops (L-l, -2, and -3) are as shown. One lipid phosphatase motif, a cell adhesion sequence, a putative proton donor sequence and a dityrosine basolateral targeting sequence are as shown. Both N-and C-terminals of LPP3 protein are located inside the cytoplasm.