Figure 6
Effect of rCCN2/CTGF on the synthesis of collagen and total protein in MPL cells. Various concentrations of rCCN2/CTGF were added to confluent cultures of MPL cells, and the cells were cultured for 12 hrs. Then, the cells were labeled with [3H] proline for another 12 hrs, after which their cell layers were collected for analysis. The radioactivities of [3H] proline incorporated into total nascent proteins and collagenase-digestable portions were measured as described in "Materials and Methods." Slashed and closed boxes indicate the collagen and total protein synthesis, respectively. Values represent the mean average ± SD (n = 2). Asterisks denote statistically significant differences (**p < 0.01, *p < 0.05) from the vehicle-treated control, except the double asterisks specifying a significant difference between the stimulation levels of total protein and collagen synthesis (as indicated by the bracket). Data were computed with the results of 2 independent series of experiments with multiple sample numbers.