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Figure 4 | Cell Communication and Signaling

Figure 4

From: Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament-derived cells

Figure 4

Effects of rCCN2/CTGF on mRNA expression of osteoblast and PDL-related markers in MPL cells. Confluent cultures of MPL cells were treated with rCCN2/CTGF (100 ng/ml, closed bars) or vehicle (dotted bars) for 24 hrs. Total RNA was purified, and 1 μg of each sample was reverse-transcribed and used for PCR. Alkaline phosphatase (ALPase), type I collagen, and periostin mRNAs were shown to be up-regulated clearly by the addition of rCCN2/CTGF under exponential conditions (25, 30, and 25 cycles, respectively) for amplification. The expression of GAPDH, used for an internal control, was almost the same among these samples.

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Cell Communication and Signaling

ISSN: 1478-811X