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Figure 3 | Cell Communication and Signaling

Figure 3

From: Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament-derived cells

Figure 3

(A) Effect of rCCN2/CTGF on DNA synthesis in MPL cells. MPL cells were inoculated at a density of 5 × 103/well in 96-multi-well plates and cultured in α-MEM with 10% FBS for 2 days. Then, the culture medium was changed to α-MEM with 0.5% FBS, and the cells were incubated with various concentrations of rCCN2/CTGF for 24 hrs and labeled with [3H] thymidine for the last 4 hrs. The incorporated radioactivity was determined by a liquid scintillation counter. Values represent the averages ± SD. Data were computed with the results of 2 independent series of experiments with multiple sample numbers. Asterisks denote statistically significant difference from the vehicle-treated control. (B) Effect of rCCN2/CTGF on the proliferation of MPL cells. MPL cells were inoculated at a density of 2 × 103/well in 96-multi-well plates and cultured in α-MEM with 10% FBS for 24 hrs. Then, the medium was changed to α-MEM with 1% FBS, and the cells were cultured with various concentrations of rCCN2/CTGF for 3 days. The cell number was computed by using Tetra-Color One as specified by the manufacturer. Points and bars represent the averages and SD, respectively. Asterisks indicate significant differences between vehicle-treated control and rCCN2/CTGF-treated samples at the significance level of **p < 0.01 or *p < 0.05.

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