Figure 2From: Effect of connective tissue growth factor (CCN2/CTGF) on proliferation and differentiation of mouse periodontal ligament-derived cellsRT-PCR analysis of the expression of CCN2/CTGF mRNA in sparse (S) and confluent (C) MPL cultures. Total RNA was purified from MPL cultures under sparse and confluent conditions, and 1 μg in each sample was reverse transcribed and used for PCR. The expression of CCN2/CTGF mRNA was stronger under the sparse than the confluent condition as evaluated in the exponential phase of the amplification (25 cycles). Levels of GAPDH mRNA, used as an internal control, were almost the same among these samples.Back to article page