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Figure 6 | Cell Communication and Signaling

Figure 6

From: Regulation of trophoblast beta1-integrin expression by contact with endothelial cells

Figure 6

Western blot analysis of β1 integrin expression in trophoblasts cultured with recombinant adhesion molecules. Trophoblasts were cultured for 24 h in dishes precoated with BSA or recombinant adhesion molecules as described in Methods. The cells were then subjected to Western blot analysis using an antibody against β1 integrin. The results of a typical chemiluminescence detection assay are shown in (A). The graph in (B) shows the results of densitometric analyses of the integrin bands from three experiments. The asterisk indicates a value that is significantly different (p < 0.05) from the BSA control culture. While values for PECAM-1 and αVβ3 were significantly different from the control they were not significantly different from each other. The lane labeled Ig shows the result of incubating the blot with control mouse immunoglobulin instead of anti-β1 integrin antibody.

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