Time course of enhanced COX-2 protein expression by VEGF in HIMEC. VEGF (50 ng/ml) resulted in a maximal increase of COX-2 protein at 3 hr which declined after 24 hr as detected by western blotting. There were no changes in COX-1 protein expression in VEGF stimulated HIMEC. Pretreatment of HIMEC with 0.1 μM CsA diminished COX-2 protein expression following VEGF. Both NS398 and PD098059 were strong inhibitors of COX-2 protein expression in VEGF stimulated HIMEC (not shown).