Figure 4
Differential brain ECAM expression by metabolically stressed brain endothelial, neuronal and astrocyte secreted factors. A. Comparison of brain endothelial ICAM-1 expression. A significant increase in brain endothelial ICAM-1 expression was observed with CM obtained from astrocytes and neurons from all conditions compared to respective brain endothelial CM. B. Comparison of brain endothelial VCAM-1 expression. While no significant increase in brain endothelial VCAM-1 expression was observed with any of neuronal CM, hypoxic and OGDR astrocytic CM significantly increased brain endothelial VCAM-1 expression compared to respective brain endothelial CM. C. Comparison of brain endothelial MAdCAM-1 expression. No significant difference in brain endothelial MAdCAM-1 expression was observed with CM obtained from astrocytes and neurons from any of conditions compared to respective brain endothelial CM. D. Comparison of brain endothelial PECAM-1 expression. While no significant difference in brain endothelial MAdCAM-1 expression was observed with any of neuronal CM, astrocytic CM from all conditions significantly increased brain endothelial PECAM-1 expression compared to respective brain endothelial CM. E. Comparison of brain endothelial E-selectin expression. A significant increase in brain endothelial E-selectin was observed with both normal and aglycemic neuronal and astrocytic CM compared to respective brain endothelial CM. However, while neither hypoxic nor OGDR neuronal CM increased brain E-selectin expression, both hypoxic and OGDR astrocytic CM significantly increased E-selectin expression compared to respective brain endothelial CM. F. Comparison of brain endothelial P-selectin expression. Except OGDR astrocytic CM, none of the other conditions significantly induced brain endothelial P-selectin expression compared to respective brain endothelial CM. Un-paired t-test was used to check significance between 2 specific groups. *P < 0.05 considered significantly different from controls.