Figure 3

Differential effects of metabolic stresses on brain endothelial, neurovascular and gliovascular-leukocyte adhesive interactions. A. Effect of metabolic stresses on brain endothelial-lymphocyte adhesion. Hypoxic and OGDR treated brain endothelial cells significantly promoted lymphocyte adhesion compared to untreated and aglycemic brain endothelial cells. B. Effect of metabolic stresses on neurovascular-lymphocyte adhesion. Similar to brain endothelial-lymphocyte adhesion, hypoxia and OGDR significantly induced neurovascular-lymphocyte adhesion compared to untreated and aglycemic neurovascular-lymphocyte adhesion. C. Effect of metabolic stresses on gliovascular-lymphocyte adhesion. Similar to brain endothelial and neurovascular-lymphocyte adhesion, hypoxia and OGDR significantly induced gliovascular-lymphocyte adhesion compared to untreated and aglycemic gliovascular-lymphocyte adhesion. D. Comparison of untreated brain endothelial, neurovascular and gliovascular-lymphocyte adhesion. A significant increase in neurovascular and gliovascular-lymphocyte adhesion was observed compared to untreated brain endothelial cells. E. Comparison of aglycemic brain endothelial, neurovascular and gliovascular-lymphocyte adhesion. A significant increase in aglycemic neurovascular and gliovascular-lymphocyte adhesion was observed compared to aglycemic brain endothelial cells. F. Comparison of hypoxic brain endothelial, neurovascular and gliovascular-lymphocyte adhesion. No significant difference in hypoxic neurovascular and gliovascular-lymphocyte adhesion compared to hypoxic brain endothelial cells was observed. G. Comparison of OGDR brain endothelial, neurovascular and gliovascular-lymphocyte adhesion. A significant increase in OGDR neurovascular and gliovascular-lymphocyte adhesion was observed compared to OGDR brain endothelial cells. Un-paired t-test is used to check statistical significance between two groups. *P < 0.05 considered significantly different from controls.