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Figure 2 | Cell Communication and Signaling

Figure 2

From: Neuronal and astrocytic interactions modulate brain endothelial properties during metabolic stresses of in vitro cerebral ischemia

Figure 2

Differential effects of metabolic stresses on neuronal and astrocytic interactions in modulating brain endothelial angiogenic potential. A. Effect of metabolically stressed brain endothelial CM (self-conditioned medium) on brain endothelial angiogenesis. Significant increase in number of brain endothelial vessel like structures were observed with self-CM obtained from aglycemic, hypoxic and OGDR treated brain endothelial cells compared to untreated brain endothelial CM. B. Effect of metabolically stressed neuronal CM on brain endothelial angiogenesis. No significant difference in number of vessel like structures were observed with neuronal aglycemic, hypoxic or OGDR CM compared to untreated neuronal CM. C. Effect of metabolically stressed astrocytic CM on brain endothelial angiogenesis. Significant increase in number of vessel like structures were observed with astrocytic aglycemic and OGDR CM compared to untreated astrocyte CM. No significant differences were observed between astrocytic hypoxic CM and untreated astrocytic CM. D. Comparison of untreated CM. A significant increase in vessel like structures were observed with untreated neuronal CM compared to untreated brain endothelial CM. No differences between untreated astrocytic CM and untreated brain endothelial CM was observed. E. Comparison of aglycemic CM. No significant differences were observed between aglycemic brain endothelial, neuronal and astrocytic CM. F. Comparison of hypoxic CM. A significant decrease in vessel like structures were observed with hypoxic neuronal and astrocytic CM compared to hypoxic brain endothelial CM. G. Comparison of OGDR CM from brain endothelial cells, neurons and astrocytes. A significant decrease in vessel like structures were observed with OGDR neuronal and astrocytic CM compared to OGDR brain endothelial CM. One way ANOVA with Bonferroni post testing was used to check significance between 2 specific groups. Un-paired t-test was used to check statistical significance between two groups.*P<0.05 is considered significantly different from controls.

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