A model for the effects of DUOXA1 on muscle differentiation. The proposed model suggests that elevated levels of DUOXA1 act downstream of MyoD to initiate massive cell death and impair differentiation. DUOXA1 knockdown enhances differentiation. Samples subjected to siRNAs targeting DUOX1 or ASK1 rescues the overexpression phenotype. The effects of DUOXA1 overexpression and knockdown/rescue are presented in green and red, respectively, and suggest that DUOXA1-mediated apoptosis inhibits differentiation through a mechanism involving DUOX1 and ASK1.