Figure 5From: FTO contributes to hepatic metabolism regulation through regulation of leptin action and STAT3 signalling in liverOverexpression of FTO in liver of mice disruptsSTAT3 pathway. FTO was overexpressed in liver of mice by adenoviral infection using a recombinant adenovirus encoding human FTO or GFP (as control) proteins for 10 days (2.108ifu/g of body weight). A) Representative Western blots and quantitative analysis of pSTAT3(Y705) and SET7 proteins in nuclear fractions of liver infected with a recombinant adenovirus encoding human FTO or GFP (as control) proteins for 10 days. Controls with a marker of mitochondrial fractions illustrate that pS-STAT3 is nor present in nucleus fractions. B) Representative Western blots and quantitative analysis of pSTAT3(S727) and VDAC in mitochondrial fractions of infected liver. Controls with a marker of nucleus fractions illustrate that pY-STAT3 is nor present in mitochondrial fractions. C) mRNA levels of G6P, PEPCK, PGC1α and FOXO1α determined by real-time PCR in liver of Ad-GFP and Ad-FTO mice and expressed relative to Ad-GFP mice Data are means ± SEM (n = 4/group for A and n = 6/group for B). *p < 0.05 compared to Ad-GFP mice.Back to article page