Table 1 Primer sequences and conditions of semi-quantitative PCR
From: Mesenchymal stem cell contact promotes CCN1 splicing and transcription in myeloma cells
Gene product | Primer 5′-3′ sequence | Annealing temperature (°C) | Length of PCR product (bp) | mRNA isoform | |
|---|---|---|---|---|---|
CCN1 1-2 | Reverse | TCACCCTTCTCCACTTGACC | 56 | 470 | Intron-retained |
Forward | AGTCCTCGTTGAGCTGCTTG | 153 | Intron-free | ||
CCN1 2-3 | Reverse | ACCGCTCTGAAGGGGATCT | 56 | 502 | Intron-retained |
Forward | GGGACACAGAGGAATGCAG | 153 | Intron-free | ||
CCN1 3-4 | Reverse | GGCAGACCCTGTGAATATAA | 50 | 612 | Intron-retained |
Forward | CAGGGTTGTCATTGGTAACT | 481 | Intron-free | ||
CCN1 4-5 | Reverse | CAACCCTTTACAAGGCCAGA | 54 | 321 | Intron-retained |
Forward | TGGTCTTGCTGCATTTCTTG | 206 | Intron-free | ||
EEF1A1 | Reverse | CTGTATTGGATTGCCACG | 54 | 369 | |
Forward | AGACCGTTCTTCCACCACTG | ||||