Figure 4

CCN1-Fc enhances INA-6 cell viability in the absence of IL-6. INA-6 cells were cultured with serum-reduced media for 24 h before stimulation with (A) CCN1-Fc or (B) CCN1-Fc and IL-6. After 24 h, cell viability was measured by quantification of ATP. In the absence of IL-6, INA-6 cell viability was significantly enhanced when stimulated with 0.1 μg/ml – 1 μg/ml CCN1-Fc, whereas incubation with 0.05 μg/ml – 2.5 μg/ml CCN1-Fc led to a slight enhancement of INA-6 cell viability in presence of IL-6. Results depicted graphically represent the mean + SD for four independent experiments. *p < 0.05 relative to control.