Figure 2
From: Mesenchymal stem cell contact promotes CCN1 splicing and transcription in myeloma cells
Exogenous stimulation by recombinant CCN1-Fc induces splicing of CCN1 pre-mRNA in INA-6 cells. INA-6 cells were incubated with varying concentrations of CCN1-Fc for 24 h. Semi-quantitative PCR was performed with primers for CCN1 4-5 and EEF1A1. Densitometric analysis was used to determine the amount of intron-retaining and intron-free pre-mRNA isoform. Bar graphs showing the percentage of intron-free isoform (intron-free mRNA compared to total (intron-free + intron retained) mRNA), which is enhanced by increased concentration of CCN1-Fc. EEF1A1 was used to normalize data.