Odontoblast differentiation of DPSCs/progenitors by BBX knockdown. (A) The BBX shRNA was electroporated, and BBX expression was confirmed by RT-PCR. (B) ALP staining and mineralization was not decreased substantially by BBX shRNA (upper and middle panel). The alizarin red S stain was extracted and the OD was measured (lower graph). (C) The expression of several odontoblast differentiation markers was regulated by BBX knockdown. Note that ALP and DSPP was decreased significantly by BBX shRNA. All experiments were performed in triplicate.