Isoforms of BBX and its expression in various tissues. PCR primers were designed to detect both the total and isoforms of BBX. (A) The expression of the BBX isoforms during the odontoblast differentiation of human DPSCs/progenitors showed that variant 1 is dominantly expressed. (B) Tissue expression of Bbx was analyzed by RT-PCR. The total RNA was extracted from the tissues of adult mice, and the expression of Bbx was analyzed by RT-PCR. (C) BBX expression was also analyzed in human molar odontoblasts (DP: dental pulp, OD: odontoblast). (D) BBX was immunostained with anti-BBX antibody at day 1, 7 and 14. scale bar: 20 μm. All experiments were performed in triplicate.