Figure 4

Model validation: extracellular pathway perturbation. A). pERK time-response curves up to 2 hrs upon addition of FGF2 with (red) and without (black) soluble heparin. Experimental results are plotted as circles with standard deviations and model fits are plotted as solid curves. Note that the red curves are model predictions and not fitted to pERK response to FGF2 with heparin. B). Model prediction for pERK dose–response curve at 10 min upon addition of varying amounts of heparin into the extracellular medium. C). Experimental validation of pERK dose–response upon heparin addition to the medium.