Figure 3
NO reduced gap junctional Ca2+signal propagation in siRNA (against Cx43) treated HUVEC. A. Treatment with siRNA (24 h) against Cx43 decreased the amount of Cx43 protein in these cells. B. Depicts the time course of the Ca2+i increase in the stimulated (red), adjacent (green) and secondary adjacent (blue) cells under control conditions (top) and after application of NO (bottom, 15 min 2 μM SNAP; representative experiment). The result of all experiments (n = 21, w = 6, C = 3; *: p < 0.05) is shown in C, demonstrating a decrease of responding cells after NO treatment.