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Table 2 Cytokines detected in supernatants of FTC-133 cells after 72 h incubation

From: Spheroid formation of human thyroid cancer cells under simulated microgravity: a possible role of CTGF and CAV1

  2D Clinostat Random positioning machine
Factor LDD (pg/mL) 72 h 1g (pg/mL) 72 h s-μg (pg/mL) LDD (pg/mL) 72 h 1g (pg/mL) 72 h s-μg (pg/mL)
GM-CSF 3.2 1536 ± 166 1172 ± 31* 9.3 46.8 ± 6.9 110.8 ± 12.0*
IFN-γ 0.49 n.d. n.d. 0.58 n.d. n.d.
IL-2 1.0 1.9 ± 0.33 n.d. 2.6 n.d. n.d.
IL-3 1.1 n.d. n.d. 2.0 n.d. n.d.
IL-4 4.4 n.d. n.d. 3.2 n.d. n.d.
IL-5 1.0 n.d. n.d. 0.73 n.d. n.d.
IL-6 0.84 282 ± 37 204 ± 17* 0.85 43.8 ± 8.4 70 ± 15.6*
IL-7 2.1 n.d. 2.1 ± 0.3 5.5 n.d. n.d.
IL-8 0.63 7604 ± 410 5586 ± 267* 0.49 226 ± 31 350 ± 36*
IL-10 1.1 n.d. n.d. 1.5 n.d. n.d.
IL-18 3.7 n.d. n.d. 3.2 n.d. n.d.
MIP-1α 8.2 3086 ± 390 2198 ± 379* 6.0 113 ± 18 137 ± 24
MIP-1β 4.0 261 ± 46 166 ± 28* 4.6 n.d. n.d.
MCP-1 4.6 47 ± 6 40 ± 3 4.9 n.d. n.d.
TNF-α 2.9 8.9 ± 1.2 7.0 ± 1.3 3.1 n.d. n.d.
TNF-β 2.3 2.3 ± 0.4 n.d. 4.4 n.d. n.d.
BDNF 3.0 20 ± 1.7 16 ± 1.7* 6.2 41.2 ± 4.6 76.6 ± 4.7*
Eotaxin-1 3.7 11.8 ± 1.8 12.4 ± 2.3 13 75.4 ± 9.9 99.4 ± 9.8*
ICAM1 270 360 ± 74 350 ± 73 620 1328 ± 274 1840 ± 150*
IL-1α 0.24 n.d. n.d. 0.19 0.85 ± 0.11 1.60 ± 0.29*
IL-1β 0.3 n.d. n.d. 0.25 1.14 ± 0.22 2.02 ± 0.34*
IL-1ra 8.7 n.d. n.d. 14 127 ± 38 226 ± 24*
IL-12p40 16 n.d. n.d. 17 128 ± 21 206 ± 29*
IL-12p70 4.8 7.8 ± 0.6 7.3 ± 0.9 4.5 36.0 ± 4.2 46.2 ± 6.1
IL-15 39.0 n.d. n.d. 41 142 ± 27 208 ± 28*
IL-17 0.51 1.14 ± 0.32 1.0 ± 0.16 0.6 2.5 ± 0.4 4.5 ± 0.6*
IL-23 51 n.d. n.d. 69 590 ± 117 868 ± 78*
MMP-3 5.8 260 ± 22 256 ± 19 7.4 51.2 ± 11.3 90.8 ± 11.1*
SCF 13 24 ± 4 19 ± 2 9.0 59.0 ± 16.1 94.6 ± 11.5*
VEGF 1.4 3062 ± 539 2814 ± 309 4.2 6048 ± 791 5044 ± 677
  1. Values are given with mean ± SD; 1g, corresponding ground control; n.d., not detectable; *P <0,05 for device sample vs. corresponding 1g ground control; LDD (Least Detectable Dose)-determined as the mean ± 3 standard deviations of 20 blank readings.