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Table 2 Cytokines detected in supernatants of FTC-133 cells after 72 h incubation

From: Spheroid formation of human thyroid cancer cells under simulated microgravity: a possible role of CTGF and CAV1

 

2D Clinostat

Random positioning machine

Factor

LDD (pg/mL)

72 h 1g (pg/mL)

72 h s-μg (pg/mL)

LDD (pg/mL)

72 h 1g (pg/mL)

72 h s-μg (pg/mL)

GM-CSF

3.2

1536 ± 166

1172 ± 31*

9.3

46.8 ± 6.9

110.8 ± 12.0*

IFN-γ

0.49

n.d.

n.d.

0.58

n.d.

n.d.

IL-2

1.0

1.9 ± 0.33

n.d.

2.6

n.d.

n.d.

IL-3

1.1

n.d.

n.d.

2.0

n.d.

n.d.

IL-4

4.4

n.d.

n.d.

3.2

n.d.

n.d.

IL-5

1.0

n.d.

n.d.

0.73

n.d.

n.d.

IL-6

0.84

282 ± 37

204 ± 17*

0.85

43.8 ± 8.4

70 ± 15.6*

IL-7

2.1

n.d.

2.1 ± 0.3

5.5

n.d.

n.d.

IL-8

0.63

7604 ± 410

5586 ± 267*

0.49

226 ± 31

350 ± 36*

IL-10

1.1

n.d.

n.d.

1.5

n.d.

n.d.

IL-18

3.7

n.d.

n.d.

3.2

n.d.

n.d.

MIP-1α

8.2

3086 ± 390

2198 ± 379*

6.0

113 ± 18

137 ± 24

MIP-1β

4.0

261 ± 46

166 ± 28*

4.6

n.d.

n.d.

MCP-1

4.6

47 ± 6

40 ± 3

4.9

n.d.

n.d.

TNF-α

2.9

8.9 ± 1.2

7.0 ± 1.3

3.1

n.d.

n.d.

TNF-β

2.3

2.3 ± 0.4

n.d.

4.4

n.d.

n.d.

BDNF

3.0

20 ± 1.7

16 ± 1.7*

6.2

41.2 ± 4.6

76.6 ± 4.7*

Eotaxin-1

3.7

11.8 ± 1.8

12.4 ± 2.3

13

75.4 ± 9.9

99.4 ± 9.8*

ICAM1

270

360 ± 74

350 ± 73

620

1328 ± 274

1840 ± 150*

IL-1α

0.24

n.d.

n.d.

0.19

0.85 ± 0.11

1.60 ± 0.29*

IL-1β

0.3

n.d.

n.d.

0.25

1.14 ± 0.22

2.02 ± 0.34*

IL-1ra

8.7

n.d.

n.d.

14

127 ± 38

226 ± 24*

IL-12p40

16

n.d.

n.d.

17

128 ± 21

206 ± 29*

IL-12p70

4.8

7.8 ± 0.6

7.3 ± 0.9

4.5

36.0 ± 4.2

46.2 ± 6.1

IL-15

39.0

n.d.

n.d.

41

142 ± 27

208 ± 28*

IL-17

0.51

1.14 ± 0.32

1.0 ± 0.16

0.6

2.5 ± 0.4

4.5 ± 0.6*

IL-23

51

n.d.

n.d.

69

590 ± 117

868 ± 78*

MMP-3

5.8

260 ± 22

256 ± 19

7.4

51.2 ± 11.3

90.8 ± 11.1*

SCF

13

24 ± 4

19 ± 2

9.0

59.0 ± 16.1

94.6 ± 11.5*

VEGF

1.4

3062 ± 539

2814 ± 309

4.2

6048 ± 791

5044 ± 677

  1. Values are given with mean ± SD; 1g, corresponding ground control; n.d., not detectable; *P <0,05 for device sample vs. corresponding 1g ground control; LDD (Least Detectable Dose)-determined as the mean ± 3 standard deviations of 20 blank readings.