Figure 3

Quantitative real-time PCR for the determination of alterations in gene-expression of selected genes after 72 h. CAV1 (A, B), CAV2 (C, D), CTGF (E, F), EGF (G, H), ERK1 (I, J), IL-8 (K, L), ITGB1 (M, N), and PRKCA (O, P) gene expression was analyzed after 72 h exposure of the cells to 2-D Clinostat (A, C, E, G, I, K, M, O) or random positioning machine (RPM; B, D, F, H, J, L, N, P). After 72 h culturing, the FTC-133 cells grew adherently (AD) or within the MCTS. On both machines CAV1 (C, D) was down-regulated in the MCTS cells and CTGF (E, F) was differently expressed in AD and MCTS, respectively. All results are shown as mean ± standard deviation (SD) of n = 10 independent samples, with significance indicated by *P < 0.05 vs. 1g, **P < 0.05 vs. s-μg: AD.