Figure 3

Identification of THOC5 dependent genes in steady state and under serum stimulation conditions. A: Summary table of transcriptome analyses in THOC5 depleted Hela cells [32], mouse embryonic fibroblasts [25], and bone marrow derived macrophages [20]. B: Transcriptome analysis of serum inducible genes in mouse embryonic fibroblasts from tamoxifen inducible THOC5 knockout mice [21]. Mouse embryonic fibroblasts with or without THOC5 were serum starved for 24 h and then stimulated with serum for 1 h. Total RNA was isolated for transcriptome analysis as described previously [20]. Ninety-four genes were upregulated more than 4-fold in the presence of THOC5. In absence of THOC5, only 9 genes were induced to a similar extent. * including direct and indirect THOC5 dependent genes.