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Figure 2 | Cell Communication and Signaling

Figure 2

From: β-catenin promotes the type I IFN synthesis and the IFN-dependent signaling response but is suppressed by influenza A virus-induced RIG-I/NF-κB signaling

Figure 2

IAV infection induces nuclear accumulation of β-catenin molecules. HEK293 cells were stimulated with 100 mg/ml Wnt3a or infected with either FPV or PR8 (MOI = 5) for 6 h. Subsequently, cell lysates were fractionated and the cytosolic and nuclear fractions were analyzed by SDS-PAGE and Western blotting for β-catenin levels. (A) Immunoblotting of α-tubulin and Lamin C served as internal loading controls for cytosolic and nuclear fractions, respectively. The infection was verified by expression of viral PB2 protein. One representative Western Blot out of three is depicted. (B) Relative β-catenin protein scores of Western blots are presented. They were estimated densitometrically as the relative intensity of the appropriate protein band to the loading control and normalized to the cytoplasmic or nuclear fraction of untreated cells, respectively. Values are means of three independently repeated experiments.

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Cell Communication and Signaling

ISSN: 1478-811X