Y1198in the kinase domain of Ron regulates the autoinhibitory constraints on receptor activation imposed by the JM-C region. A) Schematic of the Ron receptor. Y1198 at the interface of the αE helix and the αC helix is highlighted. B) &D) 293 cells were transfected with HA-tagged wild-type or mutant Ron as indicated in the presence and absence of MSP. Lysates were blotted for p-Ron1238/9Y, HA, p-Erk and Erk. C) &E) Relative luciferase activity in 293 cells co-transfected with wild-type or mutant Ron as indicated, and an AP-1 luciferase reporter in the presence and absence of MSP.