Figure 3
From: Autoinhibition of the Ron receptor tyrosine kinase by the juxtamembrane domain
Serines in the JM-C region play a subsidiary role in the regulation of Ron receptor activation. A) Schematic of mutants. Mutations are highlighted by underline. B) 293 cells were transfected with HA-tagged wild-type or mutant Ron as indicated in the presence and absence of MSP. Lysates were blotted for p-Ron1238/9Y, HA, p-Erk and Erk. C) Relative luciferase activity in 293 cells co-transfected with wild-type or mutant Ron as indicated, and an AP-1 luciferase reporter in the presence and absence of MSP.