Figure 2

Acidic residues in the JM-C region of Ron promote receptor autoinhibition. A) Schematic of mutants. Mutations are highlighted in bold. Residues deleted are underlined. B) 293 cells were transfected with HA-tagged wild-type or mutant Ron as indicated in the presence and absence of MSP. Lysates were blotted for p-Ron1238/9Y, HA, p-Erk, Erk and β-tubulin. C) Relative luciferase activity in 293 cells co-transfected with wild-type or mutant Ron as indicated, and an AP-1 luciferase reporter in the presence and absence of MSP. The data were presented as the average ± standard deviation from measurements collected from three independent experiments and three measurements per experiment. Wild type Ron −/+MSP was used as controls, unless denoted with '⏠'. The significant differences of values at p < 0.05, p < 0.01, and p < 0.001 levels are indicated by *, **and ***respectively.