Figure 3
EVs enhance the angiogenic capacity of HMEC in vitro . Vessel-like structures formation by HMEC with or without EV stimulation. A: negative control DMEM FBS-free; B: positive control 20 ng/ml VEGF; C: b-EVs 1 × 105 EVs/ml; D: b-EVs 1 × 1011 EVs/ml; E: b-EVs 1 × 1015 EVs/ml; F: PDGF-EVs 1 × 1011 EVs/ml; G: quantitative analysis of vessel-like structures formation in response to varies doses of b-EVs and to PDGF-EVs (mean ± SEM, * - p < 0,05 vs. “-control”, # - p < 0,05 vs. “b-EVs, 30 μg/ml”, n = 8).