Figure 4

Differential effects of S100 proteins and TGFβ1 on NF-κB signaling. BxPC3, Capan1, MiaPaCa2 and Panc1 remained unstimulated (NC, negative control) or were stimulated for 10 min with 50 mU insulin (positive control), 0.02 ng/ml TGFβ1 alone or combined with 10 nM S100A8, 10 nM S100A9, 10 nM S100A8/A9 complex, 50 nM NT-S100A8. Western blot show IκBα phosphorylation at Ser³² site and the corresponding β-actin, used as the control. Histograms show semi-quantification of band intensities after normalization against the negative control (O.D.; ImageJ software, v 1.47). Columns indicate mean values, bars indicate SD from two independ experiments.