Figure 3

Degradation kinetics of the 2280 m/z peptide by PDAC cell conditioned media. The 2280 m/z peptide (200 μM), encompassing the 1–19 aminoacid sequence of S100A8, was incubated with BxPC3 (A and B) or Capan1 (C and D) conditioned media for 1, 2, 3, 4, 5, 6, 12 and 24 hours at 37°C before MALDI-TOF/MS analyses. The intensities of the main five peptides identified were normalized to total ion current (TIC). In non-conditioned media the abundance of the parental 19 aminoacid peptide at 2280 m/z was 0.99 both at time 0 and after 24 hours.