Figure 11

S100A8, S100A9 and TGFβ1 molecular interactions. S100A8, S100A9 and TGFβ1 and calcium ions (CaCl₂) were mixed at an equimolar concentration (11.3 μM) in an aqueous solution, incubated overnight at 37°C and then submitted for MALDI-TOF/MS analysis. A: S100A8 alone; B: S100A9 alone; C: S100A8 and S100A9; D: S100A8, S100A9 and TGFβ1; E: TGFβ1 was incubated overnight at an equimolar concentration (1.6 μM) with S100A8 and CaCl₂ at 37°C. Immunoprecipitation (IP) was performed using anti-S100A8. The immunoprecipitate underwent western blotting (WB) for the detection of TGFβ1. F: TGFβ1 was incubated overnight at an equimolar concentration with S100A9 and CaCl₂ at 37°C. Immunoprecipitation (IP) was performed using anti-S100A9. The immunoprecipitate underwent western blotting (WB) for TGFβ1 detection.