Figure 4
PGE 2 -dependent effect on proliferation behaviour. (A) Over the experimental duration of 24 hours, the number of cells changed by a fold of 4.60, 4.59, 0.86, 1.03, respectively. (B) Cell viability across treatment conditions was not significantly different. (C) Percentage of successful split ratio was 100%, 98%, 0%, 15%, 0%, and 0% respectively. The error bars represent + SEM, ***p < 0.001. Measurements represent an average of 150 cells from three independent experiments (N = 3). (D) WntA treatment resulted in an arrested state indicted by the black arrows and corresponded with a significant decrease in cell proliferation (***p < 0.001). Scale bar represents 100 μm.