Table 3 Primary antibodies used for western-blot
From: Activin A induces growth arrest through a SMAD- dependent pathway in hepatic progenitor cells
Primary antibodies | Dilusion | Manufacture |
|---|---|---|
Rabbite anti-p-samd2(Ser465/467) | 1:1000 | Cell Signaling Technology, USA |
Rabbite anti-smad2 | 1:1000 | Cell Signaling Technology, USA |
Rabbite anti-p-samd3(ser423/425) | 1:1000 | Cell Signaling Technology, USA |
Rabbite anti-smad3 | 1:1000 | Cell Signaling Technology, USA |
Rabbite anti-smad4 | 1:1000 | Epitomics, China |
Mouse anti-samd2/3 | Â | Santa Cruz Biotechnology Inc, USA |
For WB | 1:1000 | Â |
For IP | 1:50 | Â |
Rabbite anti-p-ERK1/2(Thr202/Tyr204) | 1:1000 | Cell Signaling Technology, USA |
Mouse anti-ERK1/2 | 1:2000 | Cell Signaling Technology, USA |
Rabbite anti-p-JNK1/2(Thr183/Tyr185) | 1:1000 | Cell Signaling Technology, USA |
Rabbite anti-JNK1/2 | 1:1000 | Cell Signaling Technology, USA |
Rabbite anti-p-p38(Thr180/Tyr182) | 1:1000 | Cell Signaling Technology, USA |
Rabbite anti-p38 | 1:1000 | Cell Signaling Technology, USA |
Rabbite anti-p-Rb(Ser807/811) | 1:1000 | Cell Signaling Technology, USA |
Rabbite anti-Rb | 1:1000 | Santa Cruz Biotechnology Inc, USA |
Rabbite anti-p21 | 1:1000 | Epitomics, China |
Rabbite anti-p15 | 1:1000 | Epitomics, China |
Rabbite anti-p27 | 1:1000 | Cell Signaling Technology, USA |
Rabbite anti-cyclinD1 | 1:5000 | Epitomics, China |
Mouse anti-cyclinA | 1:500 | Boster, China |
Rabbite anti-cyclinE | 1:500 | Biolegend, USA |
Rabbite anti-CDK2 | 1:500 | Santa Cruz Biotechnology Inc, USA |
Rabbite anti-CDK4 | 1:500 | Santa Cruz Biotechnology Inc, USA |
Mouse anti-GAPDH | 1:10000 | Santa Cruz Biotechnology Inc, USA |
Mouse anti-β-actin | 1:2000 | Santa Cruz Biotechnology Inc, USA |
Mouse anti-laminB | 1:500 | Proteintech Group, USA |