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Figure 2 | Cell Communication and Signaling

Figure 2

From: Activin A induces growth arrest through a SMAD- dependent pathway in hepatic progenitor cells

Figure 2

Activin A inhibited proliferation of LE6 cells. (A) 2 × 105 LE6 cells/ were seed into 6-well plate and treated with serum free LE media in the present of activin A for up to 48 hours. Cell media was harvested and concentration of TGF-β1 was detected by ELISA kit. The figure showed result of one of three independent assays (B) LE6 cells were grown in 8% FBS LE media in the present or absence of activin A (25, 50, 100, 200, 300 ng/ml) for 72 hours. Cell proliferation was assessed by CCK-8 assay. Data showed result of one of three independent assays. (C and D) LE6 cells were grown in 8% FBS LE media in the presence or absence of activin A (200 ng/ml) for 72 hours. Cell DNA synthesis was assessed by BrdU incorporation assay using FACS (C). R2 = BrdU positive cells. Cell apoptosis was assessed by AnnexinV/PI assay using FACS (D). LL = Live cells or annexin V and PI negative cells, LR = Early apoptotic cells or annexin V positive cells, UR = Late apoptotic and necrotic cells or annexin V and PI positive cells, UL = Necrotic cells or PI positive cells. Bar chart showed the apoptosis rate (UR + LR) of control and activin treated LE6 cells. The figure showed result of one of three independent assays.

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