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Figure 8 | Cell Communication and Signaling

Figure 8

From: IL-12 and IL-27 regulate the phagolysosomal pathway in mycobacteria-infected human macrophages

Figure 8

Inhibition of STAT-1 prevented the increase in phagosomal acidification during treatment with IL-12 and sIL-27R. Human macrophages were either treated with (A) DMSO as a control, (B) fludarabine, or (C) niclosamide for 6 h and then infected with BCG for an additional 48 h. Lysotracker was added at the end of infection for 1 h. (A, B, C) The images shown are representative of two independent experiments. (D) The percent association and MFI were calculated as described in the Methods section. These data are the combined results of two independent experiments ± standard error. A student’s t test was used to establish statistical significance in the 95% confidence interval between individual sample groups as indicated; *indicates that IL-12 and sIL-27R is significantly different from BCG alone (BCG). **indicates that BCG alone (BCG) is significantly different from MED alone (MED).

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