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Figure 3 | Cell Communication and Signaling

Figure 3

From: Downstream signaling and genome-wide regulatory effects of PTK7 pseudokinase and its proteolytic fragments in cancer cells

Figure 3

Genome-wide transcriptional profiling. A. Heat map and the hierarchical clustering of the expression data (log2 of expression level) of the differentially expressed genes in PTK7, sPTK7, Chz, cPTK7/726-1070, cPTK7/622-1070 and shPTK7 cells relative to the parental HT1080 cells. Red and green correspond to the high and the low expression levels, respectively (color bar inset). B. The number of differentially expressed genes in the samples. Numbers show the number of the up-regulated (red) and the down-regulated (green) genes relative to HT1080 cells. C. Heat map and the hierarchical clustering of the expression data (log2 of expression level) of the differentially expressed, cell motility-related genes in the cells (selected using the IPA software) relative to HT1080 cells (Additional file 2: Table S2). Red and green correspond to the high and the low expression levels, respectively (color bar inset). D. Expression level validation of the selected up-regulated genes by immunoblotting. Top two panels, PTK7 was analyzed using the PTK7 antibody in the total cell lysates (total) and the cell surface-biotinylated cell samples (surface). Bottom panel, The FLAG- and V5-tagged recombinant PTK7 constructs were analyzed using the FLAG and V5 antibodies (total cell lysate samples), respectively. CDH11, cadherin-11; SPP1, osteopontin; IL1B, interleukin 1β; PDPN, podoplanin; MAGEC1, melanoma antigen family C1. The numbers above the protein bands show the fold-difference of the differentially expressed genes as detected by transcriptional profiling. Tubulin, loading control.

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