Figure 7From: Intracellular signaling prevents effective blockade of oncogenic gp130 mutants by neutralizing antibodiesEffect of dominant-negative Stat3 on signaling of CAgp130. T-REx-293 cells and cells stably transfected with Stat3-Y705F-YFP were transfected with equal amounts of CAgp130-YFP. Expression of CAgp130 or CAgp130 and Stat3-Y705F was induced with 20 ng/ml dox for the indicated periods of time. TCLs were analyzed by immunoblotting using Abs against pStat3(Y705), Stat3 and gp130. The detected endogenous Stat3 serves as loading control.Back to article page