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Figure 6 | Cell Communication and Signaling

Figure 6

From: Intracellular signaling prevents effective blockade of oncogenic gp130 mutants by neutralizing antibodies

Figure 6

Effect of neutralizing gp130 Abs on signaling of CAgp130. T-REx-293-WTgp130-YFP (A) and T-REx-293-CAgp130-YFP (B) were left untreated or expression was induced with 20 ng/ml dox for the indicated periods of time. Cells were simultaneously incubated with indicated amounts of neutralizing gp130 Abs and subsequently stimulated with 200 U/ml IL-6 and 0.5 μg/ml sIL-6Rα or left unstimulated. TCLs were analyzed by immunoblotting using Abs against pStat3(Y705), Stat3, gp130 and actin as loading control.

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