Figure 5

Regulation of cerulein-induced ER stress and apoptosis by TCPTP. Total pancreas lysates from control without (n = 9) and with (n = 12) cerulein and panc-TCPTP KO without (n = 7) and with (n = 9) cerulein were immunoblotted for pPERK and peIF2α and their respective unphosphorylated proteins, cleaved Caspases 8, 9 and 3, PARP and Tubulin as a loading control. Bar graphs represent normalized data for pPERK/PERK, peIF2α/eIF2α and Caspase 8, 9, 3 and PARP/Tubulin as means ± SEM. (*: P ≤ 0.05; **: P ≤ 0.01) indicates significant difference between saline- and cerulein-injected mice, and (#: P ≤ 0.05; ##: P ≤ 0.01) indicates significant difference between WT and KO mice.