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Figure 5 | Cell Communication and Signaling

Figure 5

From: ICAM-2 regulates vascular permeability and N-cadherin localization through ezrin-radixin-moesin (ERM) proteins and Rac-1 signalling

Figure 5

In vitro and in vivo cell permeability assays. A- Cells were grown in full medium on gold electrodes until stable impedance was reached. TEER changes were recorded after 48 h and changes in TEER at baseline or B- after 10 min thrombin stimulation. Shown are average values -/+ s.e.m. of 4 independent experiments. C- Cells were grown in full medium on gold electrodes until stable impedance was reached. Cells were then infected with recombinant adenovirus encoding constitutively active (DA) or dominant negative (DN) myc-Rac1 at an m.o.i. of 400. After 4 h the virus was removed and cells starved to assess barrier development. TEER changes were recorded after 48 h. Shown are average values -/+ s.e.m of 4 independent experiments. D- In vivo permeability assay: albumin leakage in postcapillary venules from the cremaster muscles of WT and ICAM-2 KO (IC2 neg) mice in response to thrombin (2U/mL) during 10 min. Data are mean ± s.e.m. of n = 6 mice per group. t-test WT vs IC2-/- *p < 0.05, **p < 0.01.

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