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Figure 1 | Cell Communication and Signaling

Figure 1

From: ICAM-2 regulates vascular permeability and N-cadherin localization through ezrin-radixin-moesin (ERM) proteins and Rac-1 signalling

Figure 1

Distribution of ICAM-2, VECad and NCad in sub-confluent vs confluent HUVEC monolayers. Cells were seeded at low confluence (10000 cells/cm2) and cultured for 24 and 96 hours to achieve sub confluent and confluent endothelial monolayer respectively. For NCad/VECad co-staining (a-f), NCad was stained using mAb Cl32 anti-NCad followed by anti-mouse AlexaFluor488 (Green) and VECad was stained using mAb Cl55-7H1 anti-human VECad prelabelled with the Zenon® mouse IgG1 555 kit (Red). For ICAM-2/VECad co-staining (g-l), ICAM-2 was visualized using mAb BT-1 followed by anti-mouse AlexaFluor 488 (Green) and VECad was stained as described above. Co-staining for ICAM-2 and NCad was attempted with several antibodies and methods, however due to species incompatibility and the well known limitations of NCad antibodies available, satisfactory images could not be achieved. Bar = 25 μm.

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