Figure 3
From: Hepatic Hedgehog signaling contributes to the regulation of IGF1 and IGFBP1 serum levels
Expression changes of Hedgehog signaling components. qRT-PCR analyses of hedgehog members in hepatocytes freshly isolated from 12-week-old male SAC-WT (white bars) (n = 8-17) and SAC-KO (black bars) (n = 8-13) mice: (A): Smo, Ihh and Shh; (B): Boc, Cdo and Ptch1; (C): Hhip1, Fu and Sufu, and the transcription factors (D): Gli1, Gli2 and Gli3. Values are presented as relative means ± SEM; *, p < 0.05, **, p < 0.01. Immunohistochemical staining of (E): Indian Hedgehog and (F): GLI3 in liver sections from male transgenic SAC-WT and SAC-KO mice at the age of 12 weeks. Scale bars: 100 μM; 50 μM and 25 μM.