Figure 2
From: Hepatic Hedgehog signaling contributes to the regulation of IGF1 and IGFBP1 serum levels
Expression of markers for non-parenchymal cells in transgenic SAC mice. qRT-PCR analyses of (A): Acta2, Gfap and Emr1; (B): Pkm2, Krt19 and Cd34 in liver tissue of 12 weeks old male SAC-WT (white bars) (n = 13) and SAC-KO (black bars) (n = 11) mice. Values are presented as the means ± SEM. Immunohistochemistry in liver sections from male SAC-WT and SAC-KO mice of (C): GFAP (present only in HSC’s), (D): PKM2 (Is present in all non-parenchymal cell types. Hepatocytes in SAC-KO mice show a very light cytoplasmic staining). Bar: 100 μm and 20 μM.