Figure 1
From: NHERF2 is crucial in ERM phosphorylation in pulmonary endothelial cells
EBP50 and NHERF2 have distinct interacting partners in EC. (A) EBP50 or NHERF2 was immunoprecipitated from lysates of BPAEC cells. Total cell lysate (input) and IP complexes were probed for ERM, EBP50 and NHERF2 specific antibodies. (B) Anti-c-myc antibody was utilized for immunoprecipitations form pCMV-myc ezrin (EZR), pCMV-myc radixin (RDX) or pCMV-myc moesin (MSN) transfected BPAEC cell lysates as described in Methods. Total cell lysates (input) and IP complexes were probed for c-myc tag, EBP50 and NHERF2. Ø AB: control of IP from BPAEC without the addition of antibody. Shown are representative data of at least 3 independent experiments.