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Table 1 Influence of three different clinorotation profiles in comparison to 1 g and 1.8 g on the ROS production determined by luminol kinetics

From: The oxidative burst reaction in mammalian cells depends on gravity

   AUC Maximum Time to maximum
1 g Mean 8750669848 4946100 1462
  ±SE 1151580523 101211 255
  n 15 15 15
60stop Mean 3784575841*** 2476726*** 2377***
  ±SE 389686291 411176 188
  n 5 5 5
Stop60 Mean 9807343407 5211433 1382
  ±SE 492287332 419108 121
  n 5 5 5
PF profile Mean 9241240447 4826909 1413
  ±SE 935625878 518891 53
  n 5 5 5
1.8 g Mean 18867748089*** 11732458*** 2338***
  ±SE 2654166856 442477 252
  n 5 5 5
  1. Cells were activated by opsonized zymosan stimulation beforehand.Luminescence was measured in counts per second (CPS) and quantified by calculating the area under the curves (AUC, total ROS production), the maximum peak height (Maximum) and the time to the maximum peak hight (Time to maximum). Values of normalized data are listed as means ± standard error (SE). n, number of replicates; 60Stop, 60 rpm for 20 min followed by 1 g for 30 min; Stop60, 1 g for 20 min followed by 60 rpm for 30 min; PF profile, simulation of gravitational changes during a parabolic flight; ***p < 0.001.