Figure 8
From: The oxidative burst reaction in mammalian cells depends on gravity
ROS production during clinorotation, phagocytosis and sedimentation. A. Influence of clinorotation on the phagocytosis-mediated and phagocytosis-independent ROS production determined by the NBT assay. Cells were incubated with NBT-zymosan (white bars) or NBT-PBS (black bars) and directly subjected to rotation at 60 rpm for the indicated time periods. After rotation, ROS production was measured in relative optical density (OD) at 630 nm using a microplate reader. B. Influence of clinorotation and sedimentation on the phagocytosis-mediated ROS production determined by the NBT assay. Cells were incubated with NBT-zymosan (white bars) with or without methyl cellulose (MC) and directly subjected to rotation at 60 rpm for 60 min. Sedimentation effects in control cells were minimized using a supplementation of 0.3% MC. After rotation, ROS production was measured in relative optical density (OD) at 630 nm using a microplate reader. **p<0.01; ***p<0.001.