Figure 11
From: The oxidative burst reaction in mammalian cells depends on gravity
Influence of centrifugation on the Multi Sample Incubator Centrifuge on the phagocytosis-mediated superoxide production determined by the NBT assay. Cells were incubated with NBT-zymosan and directly subjected to rotation at 1.8 g and 3 g for 45 min. After centrifugation, ROS production was measured in relative optical density (OD) at 630 nm using a microplate reader (Promega). *p<0.05.