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Figure 5 | Cell Communication and Signaling

Figure 5

From: The cell-to-cell coordination between activated T cells and CpG-stimulated macrophages synergistically induce elevated levels of IL-10 via NF-κB1, STAT3, and CD40/CD154

Figure 5

Activation or inhibition of CD40/CD154 pathway in the presence of CD3/CD28/CpG acts as a switch in the expression of IL-10 or IL-30. (A) Supernatants from wildtype or CD40-/-splenocytes were treated with CD3/CD28/CpG for 72 hours were analyzed for IL-10 production by ELISA. (B) Supernatants from wild type or CD154-/- splenocytes treated with CD3/CD28/CpG for 72 hours were analyzed for IL-10 production by ELISA. (C, D) Splenocytes from wild type mice were treated with the indicated treatments in the presence of control or anti-CD40 antibodies for 72 hours, and IL-10 (C) or IL-30 (D) expression was measured in the supernatants using ELISA. (E, F) Splenocytes from wild type mice were treated with the indicated treatments in the presence of control or anti-CD40 antibodies for 24, 48, or 72 hours, and IL-10 (E) or IL-30 (F) expression was measured in the supernatants using ELISA. *, P <0.05. N = 3.

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Cell Communication and Signaling

ISSN: 1478-811X