Figure 6From: Smad3 is required for the survival of proliferative intermediate progenitor cells in the dentate gyrus of adult miceSmad3 deficiency leads to apoptotic cell death and fewer intermediate progenitor cells. (A) The total number of BrdU-ir cells 30 minutes, 8 hours and 24 hours after delivering the BrdU pulse (150 mg/Kg: **, P ≤ 0.01, two-way ANOVA, Holm-Sidak post hoc test n = 3-5 mice per genotype). (B) Histograms showing the total number of activated caspase 3-ir cells and those double-labeled with BrdU in the DG (*, P ≤ 0.05, **, P ≤ 0.01, Student t-test, n = 3 mice per genotype). (C) Confocal images of activated caspase 3/BrdU double-labeling, 24 hours after BrdU injection showing apoptotic BrdU-ir cells in the DG. Scale bar 10 μm. (D) Confocal image showing nuclear activated caspase 3 in intermediate progenitor cells expressing Mash1 in the cytoplasm. Cell shrinkage morphology, nuclear condensation and fragmentation and activated caspase 3 are suggestive of apoptosis. Scale bar 10 μm. (E-F) Quantification of the number of Mash1(+) cells in the DG along the rostro-caudal axis showed that Smad3+/+ mice had fewer intermediate progenitor cells (**, P ≤ 0.01, Student t-test, n = 6 per genotype).Back to article page